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elisa kits  (R&D Systems)
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Fig. 5 AGE stimulates fibroblasts to promote cellular migration and invasion in <t>a</t> <t>RAGE</t> dependent manner. A RAGE mRNA stromal levels in normal and invasive breast cancer cases from Oncomine. B <t>ELISA</t> (left panel) and qPCR (right panel) analysis of RAGE levels in primary mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. C qPCR analysis of mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. D Schematic representation of two compartment transwell co-culture assay. Transwell migration assay of HC11 E and Met1 F cells co-cultured with either no fibroblasts or fibroblasts isolated from mice fed a regular (REG) or high AGE (HIGH) diet. Fibroblasts in the lower chamber were also either untreated or treated with AGE ex vivo. Transwell migration (G) and invasion (H) of Met1 epithelial cells co-cultured with fibroblasts isolated from RAGE +/+ (regular n = 2; high AGE n = 2) or RAGE-/- (regular n = 2; high AGE n = 2) mice, and either untreated or treated with ex vivo AGE. Technical Replicates (n = 2); Biological Replicates (n = 3). Values are mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001
Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 5 AGE stimulates fibroblasts to promote cellular migration and invasion in <t>a</t> <t>RAGE</t> dependent manner. A RAGE mRNA stromal levels in normal and invasive breast cancer cases from Oncomine. B <t>ELISA</t> (left panel) and qPCR (right panel) analysis of RAGE levels in primary mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. C qPCR analysis of mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. D Schematic representation of two compartment transwell co-culture assay. Transwell migration assay of HC11 E and Met1 F cells co-cultured with either no fibroblasts or fibroblasts isolated from mice fed a regular (REG) or high AGE (HIGH) diet. Fibroblasts in the lower chamber were also either untreated or treated with AGE ex vivo. Transwell migration (G) and invasion (H) of Met1 epithelial cells co-cultured with fibroblasts isolated from RAGE +/+ (regular n = 2; high AGE n = 2) or RAGE-/- (regular n = 2; high AGE n = 2) mice, and either untreated or treated with ex vivo AGE. Technical Replicates (n = 2); Biological Replicates (n = 3). Values are mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001
Mouse Rage Duoset Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 5 AGE stimulates fibroblasts to promote cellular migration and invasion in <t>a</t> <t>RAGE</t> dependent manner. A RAGE mRNA stromal levels in normal and invasive breast cancer cases from Oncomine. B <t>ELISA</t> (left panel) and qPCR (right panel) analysis of RAGE levels in primary mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. C qPCR analysis of mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. D Schematic representation of two compartment transwell co-culture assay. Transwell migration assay of HC11 E and Met1 F cells co-cultured with either no fibroblasts or fibroblasts isolated from mice fed a regular (REG) or high AGE (HIGH) diet. Fibroblasts in the lower chamber were also either untreated or treated with AGE ex vivo. Transwell migration (G) and invasion (H) of Met1 epithelial cells co-cultured with fibroblasts isolated from RAGE +/+ (regular n = 2; high AGE n = 2) or RAGE-/- (regular n = 2; high AGE n = 2) mice, and either untreated or treated with ex vivo AGE. Technical Replicates (n = 2); Biological Replicates (n = 3). Values are mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001
Srage, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 5 AGE stimulates fibroblasts to promote cellular migration and invasion in <t>a</t> <t>RAGE</t> dependent manner. A RAGE mRNA stromal levels in normal and invasive breast cancer cases from Oncomine. B <t>ELISA</t> (left panel) and qPCR (right panel) analysis of RAGE levels in primary mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. C qPCR analysis of mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. D Schematic representation of two compartment transwell co-culture assay. Transwell migration assay of HC11 E and Met1 F cells co-cultured with either no fibroblasts or fibroblasts isolated from mice fed a regular (REG) or high AGE (HIGH) diet. Fibroblasts in the lower chamber were also either untreated or treated with AGE ex vivo. Transwell migration (G) and invasion (H) of Met1 epithelial cells co-cultured with fibroblasts isolated from RAGE +/+ (regular n = 2; high AGE n = 2) or RAGE-/- (regular n = 2; high AGE n = 2) mice, and either untreated or treated with ex vivo AGE. Technical Replicates (n = 2); Biological Replicates (n = 3). Values are mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001
Mouse Rage Duoset R Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 5 AGE stimulates fibroblasts to promote cellular migration and invasion in <t>a</t> <t>RAGE</t> dependent manner. A RAGE mRNA stromal levels in normal and invasive breast cancer cases from Oncomine. B <t>ELISA</t> (left panel) and qPCR (right panel) analysis of RAGE levels in primary mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. C qPCR analysis of mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. D Schematic representation of two compartment transwell co-culture assay. Transwell migration assay of HC11 E and Met1 F cells co-cultured with either no fibroblasts or fibroblasts isolated from mice fed a regular (REG) or high AGE (HIGH) diet. Fibroblasts in the lower chamber were also either untreated or treated with AGE ex vivo. Transwell migration (G) and invasion (H) of Met1 epithelial cells co-cultured with fibroblasts isolated from RAGE +/+ (regular n = 2; high AGE n = 2) or RAGE-/- (regular n = 2; high AGE n = 2) mice, and either untreated or treated with ex vivo AGE. Technical Replicates (n = 2); Biological Replicates (n = 3). Values are mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001
Mouse Rage Duoset Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 5 AGE stimulates fibroblasts to promote cellular migration and invasion in <t>a</t> <t>RAGE</t> dependent manner. A RAGE mRNA stromal levels in normal and invasive breast cancer cases from Oncomine. B <t>ELISA</t> (left panel) and qPCR (right panel) analysis of RAGE levels in primary mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. C qPCR analysis of mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. D Schematic representation of two compartment transwell co-culture assay. Transwell migration assay of HC11 E and Met1 F cells co-cultured with either no fibroblasts or fibroblasts isolated from mice fed a regular (REG) or high AGE (HIGH) diet. Fibroblasts in the lower chamber were also either untreated or treated with AGE ex vivo. Transwell migration (G) and invasion (H) of Met1 epithelial cells co-cultured with fibroblasts isolated from RAGE +/+ (regular n = 2; high AGE n = 2) or RAGE-/- (regular n = 2; high AGE n = 2) mice, and either untreated or treated with ex vivo AGE. Technical Replicates (n = 2); Biological Replicates (n = 3). Values are mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001
Serum Elisas, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rage  (R&D Systems)
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Fig. 5 AGE stimulates fibroblasts to promote cellular migration and invasion in <t>a</t> <t>RAGE</t> dependent manner. A RAGE mRNA stromal levels in normal and invasive breast cancer cases from Oncomine. B <t>ELISA</t> (left panel) and qPCR (right panel) analysis of RAGE levels in primary mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. C qPCR analysis of mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. D Schematic representation of two compartment transwell co-culture assay. Transwell migration assay of HC11 E and Met1 F cells co-cultured with either no fibroblasts or fibroblasts isolated from mice fed a regular (REG) or high AGE (HIGH) diet. Fibroblasts in the lower chamber were also either untreated or treated with AGE ex vivo. Transwell migration (G) and invasion (H) of Met1 epithelial cells co-cultured with fibroblasts isolated from RAGE +/+ (regular n = 2; high AGE n = 2) or RAGE-/- (regular n = 2; high AGE n = 2) mice, and either untreated or treated with ex vivo AGE. Technical Replicates (n = 2); Biological Replicates (n = 3). Values are mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001
Rage, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 5 AGE stimulates fibroblasts to promote cellular migration and invasion in a RAGE dependent manner. A RAGE mRNA stromal levels in normal and invasive breast cancer cases from Oncomine. B ELISA (left panel) and qPCR (right panel) analysis of RAGE levels in primary mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. C qPCR analysis of mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. D Schematic representation of two compartment transwell co-culture assay. Transwell migration assay of HC11 E and Met1 F cells co-cultured with either no fibroblasts or fibroblasts isolated from mice fed a regular (REG) or high AGE (HIGH) diet. Fibroblasts in the lower chamber were also either untreated or treated with AGE ex vivo. Transwell migration (G) and invasion (H) of Met1 epithelial cells co-cultured with fibroblasts isolated from RAGE +/+ (regular n = 2; high AGE n = 2) or RAGE-/- (regular n = 2; high AGE n = 2) mice, and either untreated or treated with ex vivo AGE. Technical Replicates (n = 2); Biological Replicates (n = 3). Values are mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001

Journal: Breast cancer research : BCR

Article Title: Pubertal exposure to dietary advanced glycation end products disrupts ductal morphogenesis and induces atypical hyperplasia in the mammary gland.

doi: 10.1186/s13058-023-01714-4

Figure Lengend Snippet: Fig. 5 AGE stimulates fibroblasts to promote cellular migration and invasion in a RAGE dependent manner. A RAGE mRNA stromal levels in normal and invasive breast cancer cases from Oncomine. B ELISA (left panel) and qPCR (right panel) analysis of RAGE levels in primary mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. C qPCR analysis of mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. D Schematic representation of two compartment transwell co-culture assay. Transwell migration assay of HC11 E and Met1 F cells co-cultured with either no fibroblasts or fibroblasts isolated from mice fed a regular (REG) or high AGE (HIGH) diet. Fibroblasts in the lower chamber were also either untreated or treated with AGE ex vivo. Transwell migration (G) and invasion (H) of Met1 epithelial cells co-cultured with fibroblasts isolated from RAGE +/+ (regular n = 2; high AGE n = 2) or RAGE-/- (regular n = 2; high AGE n = 2) mice, and either untreated or treated with ex vivo AGE. Technical Replicates (n = 2); Biological Replicates (n = 3). Values are mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001

Article Snippet: Mouse RAGE levels were determined using ELISA kits from R&D Systems (Minneapolis, MN; #DY1179) and performed as per the manufacturers’ instructions.

Techniques: Migration, Enzyme-linked Immunosorbent Assay, Isolation, Co-culture Assay, Transwell Migration Assay, Cell Culture, Ex Vivo